Random Amplified Polymorphic DNA (RAPDs)
- 94 Downloads
Because polymorphisms are noted by the presence or absence of amplification products from a single allele, the random amplified polymorphic DNA (RAPD) technique tends to provide only dominant markers. Individuals containing two copies of one allele are not distinguished by amplification (1,2) from those with only one copy. Dominant markers provide little linkage information for markers linked in repulsion. Therefore, when mapping, it is advisable to only work with markers linked in coupling, e.g., in a backcross or recombinant-inbred population, haploid, or gametophytic tissue or, alternately, in an F2 population with markers amplified from only one parent.
KeywordsDominant Marker Isogenic Line Bulk Segregant Analysis Linkage Information Gametophytic Tissue
- 5.Michelmore, R. W., Paran, I., and Kesseli, R. V. (1991) Identification of markers linked to disease resistance by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc. Natl. Acad. Sci. USA 88, 9828–9832.PubMedCrossRefGoogle Scholar
- 7.Reiter, R. S., Feldman, K. A., Williams, J. G. K., Rafalski, J. A., Tingey, S. V., and Scolnik, P. A. (1992) Genetic linkage of the arabidopsis genome: methods for mapping with recombinant inbreds and random amplified polymorphic DNAS. Proc. Natl. Acad. Sci. USA 89, 1477–1481.PubMedCrossRefGoogle Scholar