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Random Amplified Polymorphic DNA (RAPDs)

  • Scott Tingey
Protocol
  • 94 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Because polymorphisms are noted by the presence or absence of amplification products from a single allele, the random amplified polymorphic DNA (RAPD) technique tends to provide only dominant markers. Individuals containing two copies of one allele are not distinguished by amplification (1,2) from those with only one copy. Dominant markers provide little linkage information for markers linked in repulsion. Therefore, when mapping, it is advisable to only work with markers linked in coupling, e.g., in a backcross or recombinant-inbred population, haploid, or gametophytic tissue or, alternately, in an F2 population with markers amplified from only one parent.

Keywords

Dominant Marker Isogenic Line Bulk Segregant Analysis Linkage Information Gametophytic Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Scott Tingey
    • 1
  1. 1.DuPont Agricultural ProductsWilmington

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