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Gene Isolation by Exon Trapping

  • David B. Krizman
Protocol
  • 135 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The technology of exon trapping, sometimes called exon amplification, strives to exploit the phenomenon of mRNA splicing to discover genes directly from genomic DNA. There are three distinct exon trapping methodologies that differ simply in the genomic target of interest. The original experimental design was to capture isolated 3′-splice sites residing within fragments of genomic DNA (1), whereas later approaches focused on either complete internal exons (2, 3, 4, 5) or entire 3′-terminal exons (6). The requirement of complete, intact exons as targets has proven absolutely essential, and only the trapping of complete internal or 3′-terminal exons is practical.

Keywords

Cos7 Cell Multiple Cloning Site Ligation Reaction Terminal Exon Internal Exon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • David B. Krizman
    • 1
  1. 1.Laboratory of PathologyNational Insitutes of HealthBethesda

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