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Rapid Amplification of cDNA Ends

  • David Bertioli
Protocol
  • 204 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Rapid amplification of cDNA ends (RACE) amplifies either terminal of a cDNA, even where they have unknown sequence (1,2). RACE only requires knowledge of a short sequence within the mRNA of interest. It is often used for cloning the remainder of incomplete cDNAs.

Keywords

Structural RNAs General Primer Restriction Endonuclease Site Terminal Transferase cDNA Synthesis Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • David Bertioli
    • 1
  1. 1.Institute of Arable Crop ResearchRothampstead, HarpendenUK

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