Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products

  • Anu Suomalainen
  • Ann-Christine Syvanen
Part of the Springer Protocols Handbooks book series (SPH)


The time and labor required for generating nucleotide sequence information has been significantly reduced since the development of the polymerase chain reaction (PCR). PCR allows the production of sufficient amounts of DNA template in vitro, and consequently the time-consuming cloning procedure to obtain large enough quantities of DNA for sequencing is avoided. The PCR has transformed nucleotide sequencing into a method that can be considered even in routine diagnostics for sequencing large numbers of individual samples.


Polymerase Chain Reaction Product Affinity Capture Label Mixture Typical Polymerase Chain Reaction Dideoxynucleotide Sequencing 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Anu Suomalainen
    • 1
  • Ann-Christine Syvanen
    • 2
  1. 1.Department of Human Molecular GeneticsNational Public Health InstituteHelsinkiFinland
  2. 2.Department of Medical Science and Molecular MedicineUppsala University HospitalUppsalaSweden

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