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Direct Automated Cycle Sequencing of Polymerase Chain Reaction Products

  • Susan E. Daniels
Protocol
  • 81 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The polymerase chain reaction (PCR) is well known for being a rapid and versatile method for the amplification of defined-target DNA sequences. This technique can be applied to a variety of research areas, such as the identification and typing of single nucleotide substitutions of DNA sequence polymorphisms, and genetic mapping (1, 2, 3, 4).

Keywords

Polymerase Chain Reaction Product Cycle Sequencing Microfuge Tube Single Nucleotide Substitution Guanine Cytosine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Susan E. Daniels
    • 1
  1. 1.SequanaLa Jolla, San Diego

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