Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors

  • Neil Brewis
Part of the Springer Protocols Handbooks book series (SPH)


The dideoxy chain-termination method (1) involves enzymatic elongation of a oligonucleotide primer that is annealed to a single-stranded DNA template. Single-stranded DNA of bacteriophage M13 (2) or phagemid vectors (3) give the most consistently satisfactory sequencing data. However, denatured double-stranded plasmid DNA can also serve as a suitable template and can also yield several hundred nucleotides of sequence information per reaction.


Microfuge Tube Potassium Acetate Phagemid Vector Enzymatic Elongation VCSM13 Helper Phage 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Neil Brewis
    • 1
  1. 1.Marie Curie Research InstituteOxfordUK

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