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Preparation and Analysis of DNA Sequencing Gels

  • Bimal D. M. Theophilus
Protocol
  • 163 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded products of sequencing reactions. A 4–20% polyacrylamide gel is used, normally 0.4 mm thick and at least 40 cm in length.

Keywords

Bottom Edge Straight Edge Whatman Paper Sticky Tape Molecular Weight Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Biggin, M. D., Gibson, T. J., and Hong, G. F. (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA 80, 3963–3965.PubMedCrossRefGoogle Scholar
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    Mizusawa, S., Nishimura, S., and Seela, F. (1986) Improvement of the di-deoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 14, 1319–1324.PubMedCrossRefGoogle Scholar
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    Reed, A. P., Kost, T. A., and Miller, T. J. (1986) Simple improvements in 35S dideoxy sequencing. BioTechniques 4, 306–308.Google Scholar
  4. 4.
    Wahls, W. P. and Kingzette, M. (1988) No runs, no drips, no errors: a new technique for sealing polyacrylamide gel electrophoresis apparatus. BioTechniques 6, 308–309.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Bimal D. M. Theophilus
    • 1
  1. 1.Department of HaematologyBirmingham Children’s Hospital NHS TrustBirminghamUK

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