YAC Library Screening

Hybridization and PCR-Based Screening Protocols
  • Charlotte G. Cole
  • John E. Collins
  • Ian Dunham
Part of the Springer Protocols Handbooks book series (SPH)


Yeast artificial chromosome (YAC) libraries stored in microtiter plates are available for screening as either complex PCR pools or hybridization filters generated from YACs gridded at high densities (see  Chapter 57). Different libraries may be available as either PCR pools, hybridization filters, or both. Consequently, screening strategies have been designed that rely solely on either technique, or use a combined approach. Clearly, access to the YAC library microtiter plates and an automatic gridding system allows the user greater flexibility and is an advantage in large mapping projects.


Microtiter Plate Hybridization Buffer Yeast Artificial Chromosome Primary Pool Saran Wrap 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Charlotte G. Cole
    • 1
  • John E. Collins
    • 1
  • Ian Dunham
    • 1
  1. 1.The Sanger CentreWellcome Trust Genome CampusCambridgeUK

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