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Construction and Screening of Cosmid Libraries

  • Jens Hanke
  • Jörg D. Hoheisel
Protocol
  • 189 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Cosmid libraries still are an important tool for cloning large genomic regions. Although other Escherichia coli-based vector systems like bacteriophage PI, bacterial artificial chromosomes (BACs), and PI-based artificial chromosomes (PACs) allow the cloning of longer inserts, they are more difficult to handle (1). Therefore, even if they are frequently used for the initial generation of libraries from large genomes, cosmid vectors are often still required to generate libraries with smaller, more manageable pieces or libraries from limited DNA amounts, such as bands isolated from pulsed-field gels (2).

Keywords

Bacterial Artificial Chromosome Phage Particle Cosmid Library Ligation Buffer Cosmid Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Jens Hanke
    • 1
  • Jörg D. Hoheisel
    • 1
  1. 1.Functional Genome AnalysisDeutsches KrebsforschungszentrumHeidelbergGermany

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