Production of Double-Stranded cDNA for Gene Library Synthesis

  • Jane Kirk
  • Steve Mayall
Part of the Springer Protocols Handbooks book series (SPH)


The synthesis of complementary DNA (cDNA) from an mRNA template by the action of reverse transcriptase is a fundamental technique in molecular cloning. The objective is to make a large number of long cDNA copies. The quality and length of the cDNA product largely depends on the quality of the mRNA used as the starting material. Great care must therefore be taken to avoid mRNA degradation. The choice of primers and RTase used during the synthesis also influences the final product.


Avian Myeloblastosis Virus cDNA Synthesis Reaction Avian Myeloblastosis Virus Reverse Transcriptase Synthesis Buffer Radioactive Nucleotide 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Jane Kirk
    • 1
  • Steve Mayall
    • 1
  1. 1.ICRF, Clare Hall LaboratoriesSouth MimmsUK

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