Nonradioactive Northern Blotting of RNA
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Technical improvements of methods used for the nonradioactive detection of nucleic acids have replaced the 32P-based techniques in many laboratories. The most commonly used labels are digoxigenin, fluorescein, and biotin, which are linked through a spacer to a nucleotide and are incorporated into specific probes by different methods (1, 2, 3).
KeywordsSodium Dodecyl Sodium Hybridization Solution Whatman Paper Substrate Buffer Hybridization Time
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