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Nonradioactive Northern Blotting of RNA

  • Rainer Löw
Protocol
  • 519 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Technical improvements of methods used for the nonradioactive detection of nucleic acids have replaced the 32P-based techniques in many laboratories. The most commonly used labels are digoxigenin, fluorescein, and biotin, which are linked through a spacer to a nucleotide and are incorporated into specific probes by different methods (1, 2, 3).

Keywords

Sodium Dodecyl Sodium Hybridization Solution Whatman Paper Substrate Buffer Hybridization Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Rainer Löw
    • 1
  1. 1.Zentrum Moleculare Biologie Heidelberg (ZMBH)HeidelbergGermany

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