Nonradioactive Northern Blotting of RNA

  • Rainer Löw
Part of the Springer Protocols Handbooks book series (SPH)


Technical improvements of methods used for the nonradioactive detection of nucleic acids have replaced the 32P-based techniques in many laboratories. The most commonly used labels are digoxigenin, fluorescein, and biotin, which are linked through a spacer to a nucleotide and are incorporated into specific probes by different methods (1, 2, 3).


Sodium Dodecyl Sodium Hybridization Solution Whatman Paper Substrate Buffer Hybridization Time 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Rainer Löw
    • 1
  1. 1.Zentrum Moleculare Biologie Heidelberg (ZMBH)HeidelbergGermany

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