Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA

  • François Mallet
Part of the Springer Protocols Handbooks book series (SPH)


There is a need for specific and sensitive methods of characterization of RNA adapted to clinical applications. For this purpose, we have designed a procedure consisting of a continuous reverse transcriptase-polymerase chain reaction (RT-PCR) step coupled with a nonradioactive detection on microtiter plates of amplified sequences, which we called enzyme-linked oligosorbent assay (ELOSA).


Detection Probe Capture Probe Hybridization Buffer Reverse Transcription Step Amplification Buffer 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • François Mallet
    • 1
  1. 1.CNRS-BioMerieuxLyonFrance

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