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Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA

  • François Mallet
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

There is a need for specific and sensitive methods of characterization of RNA adapted to clinical applications. For this purpose, we have designed a procedure consisting of a continuous reverse transcriptase-polymerase chain reaction (RT-PCR) step coupled with a nonradioactive detection on microtiter plates of amplified sequences, which we called enzyme-linked oligosorbent assay (ELOSA).

Keywords

Detection Probe Capture Probe Hybridization Buffer Reverse Transcription Step Amplification Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • François Mallet
    • 1
  1. 1.CNRS-BioMerieuxLyonFrance

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