Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence

  • Claire B. Wheeler
  • Bronwen M. Harvey
  • Martin Cunningham
Part of the Springer Protocols Handbooks book series (SPH)


DNA probes can be labeled using a fluorescein-labeled nucleotide and subsequently detected with a dioxetane-based chemiluminescence system using an antifluorescein antibody conjugated to alkaline phosphatase (1,2). The use of chemiluminescence avoids the fading of results associated with some colorimetric detection procedures used with some nonradioactive labeling and detection systems. Hard-copy results showing excellent resolution are produced.


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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Claire B. Wheeler
    • 1
  • Bronwen M. Harvey
    • 1
  • Martin Cunningham
    • 1
  1. 1.Research and DevelopmentAmersham Pharmacia Biotech, Ltd.UK

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