Generation of Labeled Probes by Polymerase Chain Reaction
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Nucleic acid probes are an important tool in molecular diagnosis. To facilitate the detection of hybridized probes, they are labeled with a reporter molecule, which is usually a radioisotope. For diagnostic techniques carried out in a clinical laboratory, radioisotopes are hazardous and, thus, recently there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability over time compared with isotopic probes that have limited half-lives.
KeywordsPolymerase Chain Reaction Sodium Dodecyl Sulfate Polymerase Chain Reaction Product Nitro Blue Tetrazolium Nitro Blue Tetrazolium
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