Generation of Labeled Probes by Polymerase Chain Reaction

  • Y. M. Dennis Lo
  • Shu F. An
Part of the Springer Protocols Handbooks book series (SPH)


Nucleic acid probes are an important tool in molecular diagnosis. To facilitate the detection of hybridized probes, they are labeled with a reporter molecule, which is usually a radioisotope. For diagnostic techniques carried out in a clinical laboratory, radioisotopes are hazardous and, thus, recently there is a move to use nonisotopic labels, such as biotin and digoxigenin. Nonisotopic probes also have the advantage of much better stability over time compared with isotopic probes that have limited half-lives.


Polymerase Chain Reaction Sodium Dodecyl Sulfate Polymerase Chain Reaction Product Nitro Blue Tetrazolium Nitro Blue Tetrazolium 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Y. M. Dennis Lo
    • 1
  • Shu F. An
    • 2
  1. 1.Department of Chemical Pathology, Prince of wales HospitalThe Chinese University of Hong KongHong KongPeople’s Republic of China
  2. 2.Institute of NeurologyUniversity of LondonLondonUK

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