Oligonucleotide PRINS DNA Synthesis
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The technique for labeling chromosomes by annealing an oligonucleotide DNA primer to the denatured DNA of chromosome preparations on glass slides and extending it enzymatically in situ with the incorporation of labeled nucleotides was first described by Koch et al. in 1989 (1). Since then, the technique has been greatly improved in reliability, sensitivity, and resolution, and now provides a viable, rapid alternative to conventional fluorescence in situ hybridization (FISH) for many investigations, particularly the identification of chromosome aneuploidy in metastatic tissues and antenatal diagnosis and the analysis of the human chromosome complement of somatic hybrid cell lines (2, 3, 4, 5, 6).
KeywordsAmersham Pharmacia Biotech Wash Buffer Skim Milk Powder Chromosome Aneuploidy Stop Buffer
- 9.Speel, E. J. M., Lawson, D., Ramachers, F. C. S., Gosden, J. R., and Hopman, A. H. N. (1997) Combined immunocytochemistry and PRINS DNA synthesis for simultaneous detection of phenotypic and genomic parameters in cells, in Methods in Molecular Biology, vol. 71, PRINS and In Situ PCR Protocols (Gosden, J. R., ed.), Humana, Totowa, NJ, pp. 53–59.Google Scholar
- 10.Multiple sequential oligonucleotide primed in situ DNA synthesis (MULTI-PRINS) (1997), in Methods in Molecular Biology, vol. 71, PRINS and In Situ PCR Protocols, (Gosden, J. R., ed.), Humana, Totowa, NJ, pp. 39–44.Google Scholar