Environmental Influences on Cells in Culture

  • Jane E. Bottenstein
Part of the Neuromethods book series (NM, volume 23)


The technique of culturing cells derived from the nervous system has been in use for over 80 years, with the objective of simplifying the experimental system to provide readily manipulatable models of neural function. These preparations are useful for testing hypotheses relevant to cell adhesion, motility, survival, proliferation, longevity, and expression of cell type-specific properties. Many significant modifications of these methods have resulted over the years, as new data have emerged from cell biology and neurobiology studies (see reviews by Bottenstein, 1983a,Bottenstein, 1985,Bottenstein, 1988).


Conditioned Medium Glial Fibrillary Acidic Protein B104 Cell Culture Surface Glial Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Bottenstein J. (1983a) Growth requirements of neural cells in vitro. Adv. Cell. Neurobiol. 4, 333–379.Google Scholar
  2. 2.
    Bottenstein J. (1983b) Defined media for dissociated neural cultures, in Current Methods in Cellular Neurobiology (Barker J. and McKelvy J, eds.), John Wiley, New York, pp. 107–130.Google Scholar
  3. 3.
    Bottenstein J. (1984) Culture methods for growth of neuronal cell lines in defined media, in Methods in Molecular and Cell Biology, vol. 4: Methods for Serum-free Culture of Neuronal and Lymphoid Cells (Barnes D., Sirbasku D., and Sato G., eds.), Alan Liss, New York, pp. 3–13.Google Scholar
  4. 4.
    Bottenstein J. (1985) Growth and differentiation of neural cells in defined media, in Cell Culture in the Neurosciences (Bottenstein J. and Sato G., eds.), Plenum, New York, pp. 3–43.CrossRefGoogle Scholar
  5. 5.
    Bottenstein J. (1986) Growth requirements in vitro of oligodendrocyte cell lines and neonatal rat brain oligodendrocytes. Proc. Natl. Acad. Sci. USA 83, 1955–1959.PubMedCrossRefGoogle Scholar
  6. 6.
    Bottenstein J. (1988) Advances in vertebrate cell culture methods. Science 239, G42,G48.PubMedCrossRefGoogle Scholar
  7. 7.
    Bottenstein J. and Hunter S. (1990) Culture methods for oligodendrocyte cell lines and oligodendrocyte-type 2 astrocyte lineage cells, in Methods in Neurosciences, vol. 2: Cell Culture (Conn P. M., ed.), Academic, San Diego, pp. 56–75.Google Scholar
  8. 8.
    Bottenstein J. and Michler-Stuke A. (1983) Proliferation of glial-derived cell lines in serum-free defined medium, in Developing and Regenerating Vertebrate Nervous Systems, vol 6: Neurology and Neurobiology (Coates P., Markwald R., and Kenny A., eds.), Alan Liss, New York, pp. 185–189.Google Scholar
  9. 9.
    Bottenstein J. and Michler-Stuke A. (1989) Serum-free culture of dissociated neonatal rat cortical astrocytes, in A Dissection and Tissue Culture Manual of the Nervous System (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds.), Alan Liss, New York, pp. 109–111.Google Scholar
  10. 10.
    Bottenstein J. and Sato G. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. USA 76, 514–517.PubMedCrossRefGoogle Scholar
  11. 11.
    Bottenstein J. and Sato G. (1980) Fibronectin and polylysine requirement for proliferation of neuroblastoma cells in defined medium. Exp. Cell Res. 129, 361–366.PubMedCrossRefGoogle Scholar
  12. 12.
    Bottenstein J., Hunter S., and Seidel M. (1988) CNS neuronal cell line-derived factors regulate gliogenesis in neonatal rat brain cultures. J. Neurosci. Res. 20, 291–303.PubMedCrossRefGoogle Scholar
  13. 13.
    Bottenstein J., Skaper S., Varon S., and Sato G. (1980) Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium. Exp. Cell Res. 125, 183–190.PubMedCrossRefGoogle Scholar
  14. 14.
    Hughes S.M., Lillien L., Raff M., Rohrer H., and Sendtner M. (1988) Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture. Nature 335, 70–72.PubMedCrossRefGoogle Scholar
  15. 15.
    Hunter S. and Bottenstein J. (1989) Bipotential glial progenitors are targets of neuronal cell line-derived growth factors. Develop. Brain Res. 49, 33–49.CrossRefGoogle Scholar
  16. 16.
    Hunter S. and Bottenstein J. (1990) Growth factor responses of enriched bipotential glial progenitors. Develop. Brain Res. 54, 235–248.CrossRefGoogle Scholar
  17. 17.
    Hunter S. and Bottenstein J. (1991) O-2A glial progenitors from mature brain respond to CNS neuronal cell line-derived growth factors. J. Neurosci. Res. 28, 574–582.PubMedCrossRefGoogle Scholar
  18. 18.
    Kingsbury A., Gallo V., Woodhams P., and Balazs R. (1985) Survival, morphology and adhesion properties of cerebellar interneurons cultured in chemically defined and serum-supplemented medium. Develop. Brain Res. 17, 17–25.CrossRefGoogle Scholar
  19. 19.
    Marangos P. and Schmechel D. (1987) Neuron specific enolase, a clinically useful marker for neurons and neuroendocrine cells. Ann. Rev. Neurosci. 10, 269–295.PubMedCrossRefGoogle Scholar
  20. 20.
    Michler-Stuke A. and Bottenstein, J. (1982a) Proliferation of glial-derived cells in defined media. J. Neurosci. Res. 7, 215–228.PubMedCrossRefGoogle Scholar
  21. 21.
    Michler-Stuke A. and Bottenstein J. (1982b) Defined media for growth of human and rat glial-derived cell lines, in Cold Spring Harbor Conferences on Cell Proliferation, vol. 9 (Sirbasku D., Sato G., and Pardee A., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 959–971.Google Scholar
  22. 22.
    Michler-Stuke A., Wolff J.R., and Bottenstein, J. (1984) Factors influencing astrocyte growth and development in defined media. Int. J. Develop. Neurosci. 2, 575–584.CrossRefGoogle Scholar
  23. 23.
    Miller R. and Raff M. (1984) Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct. J. Neurosci. 4, 585–592.PubMedGoogle Scholar
  24. 24.
    Raff M., Miller R.H., and Noble, M. (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303, 390–396.PubMedCrossRefGoogle Scholar
  25. 25.
    Raff M., Abney E.R., and Miller R.H. (1984) Two glial cell lineages diverge prenatally in rat optic nerve. Develop. Biol. 106, 53–60.PubMedCrossRefGoogle Scholar
  26. 26.
    Wilkin G., Levi G., Johnstone S., and Riddle P. (1983) Cerebellar astroglial cells in primary culture: expression of different morphological appearances and different ability to take up [3H]D-aspartate and [3H]GABA. Develop. Brain Res. 10, 265–277.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. Totowa, New Jersey 1992

Authors and Affiliations

  • Jane E. Bottenstein
    • 1
  1. 1.Department of Human Biological Chemistry and GeneticsUniversity of Texas Medical BranchGalveston

Personalised recommendations