Advertisement

Organotypic Slice Explant Roller-Tube Cultures

  • Susan Wray
Part of the Neuromethods book series (NM, volume 23)

Abstract

Within the past decade, organ cultures have reemerged as an important complement to in vivo studies for examining regulatory mechanisms in various neuronal systems. The principle behind organ cultures is to maintain a tissue explant in a state as close as possible to that found in vivo, i.e., to preserve some aspect of the spatial, structural, and/or synaptic organization of the original tissue. As a result, such cultures are often referred to as organotypic and are valuable tools to study organized, yet relatively isolated neuronal systems.

Keywords

Tissue Slice Slice Culture Luteinizing Hormone Release Hormone Hybridization Histochemistry Tissue Chopper 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Baertschi A. J., Beny J-L., and Gähwiler B. H. (1982) Hypothalamic paraventricular nucleus is a privileged site for brain-pituitary interaction in long-term tissue culture. Nature 295,145–147.PubMedCrossRefGoogle Scholar
  2. 2.
    Baldino F., Jr. and Geller H. M. (1986) Hypothalamic explant tissue cultures in pharmacological research, in Modern Methods in Pharmacology, vol. 3, Electrophysiological Techniques in Pharmacology (Geller H. M., ed.), Liss, New York, pp. 103–119.Google Scholar
  3. 3.
    Baldino F., Jr., Higgins G. A., Moke M. T., and Wolfson B. (1985) Primary explants as a model of the hypothalamus in situ. Peptides 6,249–256.PubMedCrossRefGoogle Scholar
  4. 4.
    Baldino F., Jr. and Wolfson B. (1985) Postsynaptic actions of neurotensin on preoptic-anterior hypothalamk neurons in vitro. Brain Res. 325,161–170.PubMedCrossRefGoogle Scholar
  5. 5.
    Banker G. A. and Cowan W. M. (1977) Rat hippocampal neurons in dispersed cell culture. Brain Res. 126,397–425.PubMedCrossRefGoogle Scholar
  6. 6.
    Bornstein M. B. (1973) Organotypic mammalian central and peripheral nerve tissue, in Tissue Culture: Methods and Applications. (Kruse P. F. and Patterson M. K. eds.), Academic Press, New York, pp. 86–92.Google Scholar
  7. 7.
    Bottenstein J. E. and Sato G. (eds.) (1985) Cell Culture in the Neurosciences. Plenum Press, New York.Google Scholar
  8. 8.
    Braschler U. F., Iannone A., Spenger C, Streit J., and Lüscher H-R. (1989) A modified roller tube technique for organotypic cocultures of embryonic rat spinal cord, sensory ganglia and skeletal muscle. J. Neurosci. Methods 29,121–129.PubMedCrossRefGoogle Scholar
  9. 9.
    Clarke M. J. O., Lowry P., and Gillies G. (1987) Assessment of corticotropin-releasing factor, vasopressin and somatostatin secretion by fetal hypotha-lamic neurons in culture. Neuroendocrinolqgy 46,147–154.CrossRefGoogle Scholar
  10. 10.
    Delfs J., Friend J., Ishimoto S., and Saroff D. (1989) Ventral and dorsal horn acetylcholinesterase neurons are maintained in organotypic cultures of postnatal rat spinal cord explants. Brain Res. 488,31–42.PubMedCrossRefGoogle Scholar
  11. 11.
    Fedoroff S. and Hertz L. (eds.) (1977) Cell, Tissue and Organ Cultures in Neu-robiology. Academic Press, New York.Google Scholar
  12. 12.
    Feldman S. C., Dreyfus C. F., and Lichtenstein E. S. (1982) Somatostatin neurons in the rodent hippocampus: an in vitro and in vivo immunocytochemical study. Neurosci. Lett. 33,29–34.PubMedCrossRefGoogle Scholar
  13. 13.
    Fowler J. and Crain S. M. (1985) Long-term organotypic cultures of neonatal mouse hippocampal explants in plastic multiwell plates. Proc. Eur. Tissue Soc. 33,23.Google Scholar
  14. 14.
    Freshney R. I. (ed.) (1986) Animal Cell Culture. IRL Press, Oxford.Google Scholar
  15. 15.
    Frotscher M. and Gähwiler B. H. (1988) Synaptic organization of intracellularly stained CA3 pyramidal neurons in slice cultures of rat hippocampus. Neuroscience 24,541–551.PubMedCrossRefGoogle Scholar
  16. 16.
    Gähwiler B. H. (1980a) Labelling of neurons within CNS explants by intra-cellular injection of lucifer yellow. J. Neurdbiol. 12,187–191.CrossRefGoogle Scholar
  17. 17.
    Gähwiler B. H. (1980b) Excitatory action of opioid peptides and opiates on cultured hippocampal pyramidal cells. Brain. Res. 194,193–203.PubMedCrossRefGoogle Scholar
  18. 18.
    Gähwiler B. H. (1981a) Organotypic monolayer cultures of nervous tissue. J. Neurosci. Methods 4, 329–342.PubMedCrossRefGoogle Scholar
  19. 19.
    Gähwiler B. H. (1981b) Morphological differentiation of nerve cells in thin organotypic cultures derived from rat hippocampus and cerebellum. Proc. Roy. Soc. Lond. B. 211, 287–290.CrossRefGoogle Scholar
  20. 20.
    Gähwiler B. H. (1984a) Slice cultures of cerebellar, hippocampal and hypo-thalamic tissue. Experientia 40,235–243.PubMedCrossRefGoogle Scholar
  21. 21.
    Gähwiler B. H. (1984b) Development of the hippocampus in vitro: cell types, synapses and receptors. Neuroscience 11,751–760.PubMedCrossRefGoogle Scholar
  22. 22.
    Gähwiler B. H. (1988) Organotypic cultures of neural tissue. Trends Neurosci. 11,484 189.CrossRefGoogle Scholar
  23. 23.
    Gähwiler B. H. and Brown D. A. (1985) Functional innervation of cultured hippocampal neurones by cholinergic afferents from cocultured septal explants. Nature 313,577–579.PubMedCrossRefGoogle Scholar
  24. 24.
    Gähwiler B. H., Enz A., and Hefti F. (1987) Nerve growth factor promotes development of the rat septo-hippocampal cholinergic projection in vitro. Neurosd. Lett. 75,6–10.CrossRefGoogle Scholar
  25. 25.
    Gähwiler B. H. and Hefti F. (1984) Guidance of acetylcholinesterase-containing fibers by target tissue in cocultured brain slices. Neurosdence 13,681–689.CrossRefGoogle Scholar
  26. 26.
    Gähwiler B. H., Mamoon A. M., and Tobias C. A. (1973) Spontaneous bioelectric activity of cultured cerebellar purkinje cells during exposure to agents which prevent synaptic transmission. Brain Res. 53,71–79.PubMedCrossRefGoogle Scholar
  27. 27.
    Gähwiler B. H, Marbach P., and Baertschi A. J. (1984) The hypothalamo-hypophyseal system in culture in Neuronal Comminications (Meyer B. J. and Kramer S., eds.), A. A. Balkema, Rotterdam, pp. 145–154.Google Scholar
  28. 28.
    Gähwiler B. H., Sandoz P., and Dreifuss J. J. (1978) Neurones with synchronous bursting discharges in organ cultures of the hypothalamic supraoptic nucleus area. Brain Res. 151,245–253.PubMedCrossRefGoogle Scholar
  29. 29.
    Gainer H., Castel M., and Wray S., (1989) Immunocytochemical analysis of the suprachiasmatic nucleus in organotypic cultures. Soc. Neurosci. Abstr. 15,431.9.Google Scholar
  30. 30.
    Geller H. (1981a) Histamine actions on activity of cultured hypothalamic neurons: evidence for mediation by HI and H2-histamine receptors. Dev. Brain Res. 1,89–101.CrossRefGoogle Scholar
  31. 31.
    Geller H. (1981b) Electrophysiological pharmacology of hypothalamic neurons in explant tissue culture, in Advances in Physiological Sciences, vol. 14. (Stark E., Makara G. B., Haluze B., and Rappay G. Y. eds.), Akademiai Kiado, Budapest, pp. 107–111.Google Scholar
  32. 32.
    Geller H. M. and Woodward D. J. (1979) Synaptic organization of tuberal hypothalamus in tissue culture: Effects of electrical stimulation and blockers of synaptic transmission. Exp. Neurol. 64,535–552.PubMedCrossRefGoogle Scholar
  33. 33.
    Hamill O. P., Marty A., Neher E., Sakmann B., and Sigworth F. J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfliigers Arch. 391,85–100.CrossRefGoogle Scholar
  34. 34.
    Hösli E. and Hösli L. (1990) Autoradiographic localization of binding sites for neurotransmitters in explant culutres of rat central nervous system, in A Dissection and Tissue Culture Manual of the Nervous System. (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds.), Liss, New York, pp. 343–345.Google Scholar
  35. 35.
    Honig M. G. and Hume R. I. (1986) Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J. Cell Biol. 103,171–187.PubMedCrossRefGoogle Scholar
  36. 36.
    Katz L. C., Burkhalter A., and Dreyer W. J. (1984) Fluorescent latex microspheres as a retrograde neuronal marker for in vivo and in vitro studies of visual cortex. Nature 310,498–500.PubMedCrossRefGoogle Scholar
  37. 37.
    Lasnitzki I. (1986) Organ Culture, in Animal Cell Culture (Freshney R.I., ed), IRL Press, Oxford, pp. 149–182.Google Scholar
  38. 38.
    Lerea L. S. and McCarthy K. D. (1990) Analysis of receptor expression on cultured cells via combined receptor autoradiography and immunocytochemistry, in A Dissection and Tissue Culture Manual of the Nervous System. (Shahar A., de Vellis J., Vernadakis A., and Haber B. eds.), Liss, New York, pp. 346–350.Google Scholar
  39. 39.
    Llano I., Marty A., Johnson J. W., Ascher P., and Gähwiler B. H. (1988) Patch-clamp recording of amino acid-activated responses in “organotypic“ slice cultures. Proc. Natl. Acad. Sri. USA 85,3221–3225.CrossRefGoogle Scholar
  40. 40.
    Masurovsky E. B., Benitez H. H., and Murray M. R. (1971) Synaptic development in long-term organized cultures of murine hypothalamus. J. Comp. Neurol. 143,263–278.PubMedCrossRefGoogle Scholar
  41. 41.
    Maurer H. R. (1986) Towards chemically-defined, serum-free media for mammalian cell culture, in Animal Cell Culture: A Practical Approach (Freshney R.I., ed.), IRL Press, Oxford, pp. 13–32.Google Scholar
  42. 42.
    Morris M., Eskay R. L., and Sundberg D. K. (1986) A tissue culture model for the study of peptide synthesis and secretion from microdissected hypothalamic explants. Methods Enzymol. 124,359–371.PubMedCrossRefGoogle Scholar
  43. 43.
    Paul J. (1975) Cell and Tissue Culture. Churchill Livingstone, New York, NY.Google Scholar
  44. 44.
    Romijn H. J., de Jong B. M., and Ruijter J. M. (1988) A procedure for culturing rat neocortex explants in a serum-free nutrient medium. J. Neurosci. Methods 23,75–83.PubMedCrossRefGoogle Scholar
  45. 45.
    Sobkowicz H. M., Bleier R., and Monzain R. (1974) Cell survival and architectonic differentiation of the hypothalamic mamillary region of the newborn mouse in culture. Comp. Neurol. 155,355–376.CrossRefGoogle Scholar
  46. 46.
    Sofroniew M. V., Dreifuss J. J., and Gähwiler B. H. (1988) Slice explants of rat hypothalamus examined by immunohistochemical staining for neurohypophyseal peptides and GFAP. Brain Res. Bull. 20,669–674.PubMedCrossRefGoogle Scholar
  47. 47.
    Stern J. E., Mitchell T., Herzberg V. L, and North W. G. (1986) Secretion of vasopressin, oxytocin, and two neurophysins from rat hypothalamo-neurohypophyseal explants in organ culture. Neuroendocrinology 43,252–258.PubMedCrossRefGoogle Scholar
  48. 48.
    Toran-Allerand C. D. (1978) Culture of hypothalamic neurons: Organotypic culture. Biologie Cellulaire des Processes Neurosecretoires Hypothalamiques, Coll. Internation. du CNRS. 280,759–776.Google Scholar
  49. 49.
    Toran-Allerand C. D. (1990) Long-term Organotypic Culture of the CNS in Maximow Assemblies, in Methods in Neuroscience, vol. 2, Cell Cultures (Conn P. M., ed.), Academic Press, New York, (in press)Google Scholar
  50. 50.
    Wray S. and Hoffman G. (1986) A developmental study of the quantitative distribution of LHRH neurons within the central nervous system of postnatal male and female rats. J. Comp. Neurol. 252,522–531.PubMedCrossRefGoogle Scholar
  51. 51.
    Wray S., Gähwiler B. H., and Gainer H. (1988) Slice cultures of LHRH neurons in the presence and absence of brainstem and pituitary. Peptides 9,151–1175.CrossRefGoogle Scholar
  52. 52.
    Wray S., Zoeller R. T., and Gainer H. (1989a) Differential effects of estrogen on luteinizing hormone-releasing hormone gene expression in slice explant cultures prepared from specific rat forebrain regions. Mol. Endocrinol. 3,1197–1206.PubMedCrossRefGoogle Scholar
  53. 53.
    Wray S., Grant P., and Gainer H. (1989b) Evidence that cells expressing luteinizing hormone releasing hormone mRNA in the mouse are derived from progenitor cells in the olfactory placode. Proc. Nat. Acad. Sci. USA 86,8132–8136.PubMedCrossRefGoogle Scholar
  54. 54.
    Wray S., Kusano K., and Gainer H. (1991) Maintenance of LHRH and Oxytocin Neurons in slice explants cultured in serum-free media; effects of tetrodotoxin on gene expression. Neuroendo. 54,327–339.CrossRefGoogle Scholar
  55. 55.
    Zimmer J. and Gähwiler B. H. (1984) Cellular and connective organization of slice cultures of the rat hippocampus and fascia dentata. J. Comp. Neurol. 228,432–446.PubMedCrossRefGoogle Scholar
  56. 56.
    Zimmer J. and Gähwiler B. H. (1987) Growth of hippocampal mossy fibers: a lesion and coculture study of organotypic slice cultures. J. Comp. Neurol. 264,1–13.PubMedCrossRefGoogle Scholar
  57. 57.
    Zoeller R. T., Seeburg P. H., and Young W. S., HI. (1988) In situ hybridization histochemistry for messenger ribonucleic acid (mRNA) encoding gonadotropin-releasing hormone (GnRH): effect of estrogen on cellular levels of GnRH mRNA in female rat brain. Endocrinology 122, 2570–2577.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. Totowa, New Jersey 1992

Authors and Affiliations

  • Susan Wray
    • 1
  1. 1.Laboratory of NeurochemistryNINDS, NIHBethesda

Personalised recommendations