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Baculovirus Expression Vectors

  • Protocol
Practical Molecular Virology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 8))

Abstract

This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay. In addition, an outline of the biochemical methods routinely used to characterize the expressed protein product within the baculovirus system will be given. Throughout the experimental procedures, areas that researchers generally find technically difficult will be highlighted in the hope that this may ameliorate some of the problems encountered in using the system ab initio.

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References

  1. Smith, G E., Summers, M. D, and Fraser, M. J (1983) Production of human beta interferon in insect cells infected a baculovirus expression vector. Mol. Cell. Biol. 3, 2156–2165.

    PubMed  CAS  Google Scholar 

  2. Brown, M and Faulkner, P. (1977) A plaque assay for nuclear polyhedrosis viruses using a solid overlay. J. Gen. Virol 36, 361–364.

    Article  Google Scholar 

  3. Fung, M-C., Chiu, K Y. M., Weber, T., Chang, T.-W., and Chang, N. T. (1988) Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization. J. Virol. Methods 19, 33–42.

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  4. Sambrook, J, Fritsch, E. F, and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor, Cold Spring Harbor Laboratory, New York.

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Author information

Authors and Affiliations

  1. Department of Virology, Royal Free Hospital School of Medicine, Hampstead, London, UK

    Vincent C. Emery

Authors
  1. Vincent C. Emery
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Editor information

Editors and Affiliations

  1. Chester Beatty Laboratories, Institute of Cancer Research, London, UK

    Mary K. L. Collins

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© 1991 The Humana Press Inc., Clifton, NJ

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Emery, V.C. (1991). Baculovirus Expression Vectors. In: Collins, M.K.L. (eds) Practical Molecular Virology. Methods in Molecular Biology, vol 8. Humana Press. https://doi.org/10.1385/0-89603-191-8:309

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  • DOI: https://doi.org/10.1385/0-89603-191-8:309

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-191-3

  • Online ISBN: 978-1-59259-495-5

  • eBook Packages: Springer Protocols

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