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Baculovirus Expression Vectors

  • Vincent C. Emery
Part of the Methods in Molecular Biology book series (MIMB, volume 8)

Abstract

This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay. In addition, an outline of the biochemical methods routinely used to characterize the expressed protein product within the baculovirus system will be given. Throughout the experimental procedures, areas that researchers generally find technically difficult will be highlighted in the hope that this may ameliorate some of the problems encountered in using the system ab initio.

Keywords

Recombinant Virus Plaque Assay Transfection Mixture Extracellular Virus Radiolabeled Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Brown, M and Faulkner, P. (1977) A plaque assay for nuclear polyhedrosis viruses using a solid overlay. J. Gen. Virol 36, 361–364.CrossRefGoogle Scholar
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    Fung, M-C., Chiu, K Y. M., Weber, T., Chang, T.-W., and Chang, N. T. (1988) Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization. J. Virol. Methods 19, 33–42.PubMedCrossRefGoogle Scholar
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    Sambrook, J, Fritsch, E. F, and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor, Cold Spring Harbor Laboratory, New York.Google Scholar

Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Vincent C. Emery
    • 1
  1. 1.Department of VirologyRoyal Free Hospital School of Medicine, HampsteadLondonUK

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