Making High Titer Retroviral Producer Cells

  • Stephen J. Russell
Part of the Methods in Molecular Biology book series (MIMB, volume 8)


Retroviral vectors are uniquely suitable for high-efficiency gene transfer to a large target cell population and this (among other things) has led to the exploration of previously impractical strategies for somatic gene therapy (1,2). Thus, for example, cultured bone marrow can be used to recolonize mice with hematopoietic progenitors expressing a variety of genes after retroviral gene transfer (3), and efforts to achieve the same with human bone marrow may eventually result in effective therapies for sickle cell anemia, the thalassemias, and a variety of other single-gene disorders. The major factors limiting the success of this type of experiment are the maximum achievable concentration of recombinant virus particles, the density of virus receptors on the target cell population (which must be actively proliferating, since the processes of reverse transcription and integration are S-phase-dependent) and the emergence of replication-competent “helper virus” in the system.


Viral Titer NIH3T3 Fibroblast Helper Virus Packaging Cell Line Target Cell Population 
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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Stephen J. Russell
    • 1
  1. 1.Chester Beatty LaboratoriesInstitute of Cancer ResearchLondonUK

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