Production and Purification of Hybrid Ty-VLPs
The self-assembly properties of a protein encoded by the TYA gene of the yeast Ty element can be exploited to produce hybrid Ty-VLPs (virus-like particles) (1,2). There has been developed a series of expression vectors that allow the construction of Ty fusion genes containing protein coding sequences of interest (see Chapter 24). Many different hybrid Ty-VLPs have now been produced that carry additional proteins that range in size from 3 to 42 kDa. These include regions from human immunodeficiency virus-1 (HIV-1) env, pol, tat, rev, nef, and vif genes; influenza virus hemagglutinin; human α-interferon, feline leukemia virus env, and bovine papillomavirus El and E2 (1–5 and unpublished data).
KeywordsPasteur Pipet Yeast Nitrogen Base Glycerol Stock Sucrose Cushion Influenza Virus Hemagglutinin
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