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Production and Purification of Hybrid Ty-VLPs

  • Nigel R. Burns
  • Jacqueline E. M. Gilmour
  • Susan M. Kingsman
  • Alan J. Kingsman
  • Sally E. Adams
Part of the Methods in Molecular Biology book series (MIMB, volume 8)

Abstract

The self-assembly properties of a protein encoded by the TYA gene of the yeast Ty element can be exploited to produce hybrid Ty-VLPs (virus-like particles) (1,2). There has been developed a series of expression vectors that allow the construction of Ty fusion genes containing protein coding sequences of interest (see  Chapter 24). Many different hybrid Ty-VLPs have now been produced that carry additional proteins that range in size from 3 to 42 kDa. These include regions from human immunodeficiency virus-1 (HIV-1) env, pol, tat, rev, nef, and vif genes; influenza virus hemagglutinin; human α-interferon, feline leukemia virus env, and bovine papillomavirus El and E2 (1–5 and unpublished data).

Keywords

Pasteur Pipet Yeast Nitrogen Base Glycerol Stock Sucrose Cushion Influenza Virus Hemagglutinin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Adams, S. E, Dawson, K. M., Gull, K., Kingsman, S. M., and Kingsman, A J. (1987) The expression of hybrid Ty virus-like particles in yeast. Nature 329, 68–70.PubMedCrossRefGoogle Scholar
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    Malim, M. H., Adams, S. E., Gull, K., Kingsman, A. J., and Kingsman, S. M. (1987) The production of hybrid Ty:IFN virus-like particles in yeast. Nucleic Acids Res. 15, 7571–7580.PubMedCrossRefGoogle Scholar
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    Braddock, M., Chambers, A., Wilson, W., Esnouf, M P., Adams, S. E., Kingsman, A.J., and Kingsman, S. M. (1989) HIV-1 TAT “activates” presynthesized RNA in the nucleus. Cell 58, 269–279PubMedCrossRefGoogle Scholar
  6. 6.
    Gilmour, J. E. M., Senior, J. M., Burns, N. R., Esnouf, M. P., Gull, K., Kingsman, S. M., Kingsman, A. J., and Adams, S. E. (1989) A novel method for the purification of HIV-1 p24 protein from hybrid Ty-VLPs. AIDS 3, 717–723.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Nigel R. Burns
    • 1
  • Jacqueline E. M. Gilmour
    • 1
  • Susan M. Kingsman
    • 2
  • Alan J. Kingsman
    • 1
  • Sally E. Adams
    • 1
  1. 1.British Bio-technology Ltd.OxfordUK
  2. 2.Department of BiochemistryUniversity of OxfordOxfordUK

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