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Cell Lineage Studies Using Retroviruses

  • Jack Price
Part of the Methods in Molecular Biology book series (MIMB, volume 8)

Abstract

In order to study cell lineage in any given experimental situation, a method by which a label can be introduced into a precursor cell is required. By means of this label, the subsequent fate of the cell and its progeny can be followed. A retrovirus, as an agent of gene transfer, can be used as a genetic label, providing an indelible, heritable marker of lineage. Unlike most artificial means of gene transfer, the use of retroviruses in transferring genes into cells is both efficient and accurate. This has made retroviruses invaluable in studying cell lineage in vivo, particularly in mammalian embryos, which are too small and inaccessible to be amenable to study with conventional lineage labels.

Keywords

Magnesium Chloride Subsequent Fate Indigo Blue Bethesda Research Laboratory Genetic Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Jack Price
    • 1
  1. 1.The National Institute of Medical ResearchMill HillLondonUK

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