Abstract
Sample preparation is a very crucial step in two-dimensional (2-D) gel electrophoresis, in which the proteins of the sample must be brought into a state where they can be separated by isoelectric focusing in the first dimension. That is, they must be denatured, reduced, and solubilized, and they must be kept so during electrophoresis without changing their pI. The sample buffer for this purpose is traditionally called the lysis buffer.
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© 2005 Humana Press Inc., Totowa, NJ
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Vandahl, B.B., Christiansen, G., Birkelund, S. (2005). Preparation of Bacterial Samples for 2-D PAGE. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:019
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DOI: https://doi.org/10.1385/1-59259-890-0:019
Publisher Name: Humana Press
Print ISBN: 978-1-58829-343-5
Online ISBN: 978-1-59259-890-8
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