Abstract
The purpose of this chapter is to introduce the reader to standard procedures for quantifying the number of neural cells in microcultures. The chick embryo was chosen as the source of neural cells, because embryos can be conveniently grown in an inexpensive laboratory incubator, in large, replicate numbers with defined developmental stages. Also, chicken embryos are used by many investigators as a routine source of peripheral and central nervous system tissue. Nevertheless, the procedure outlined can be adapted to neural cells from any source.
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Further Reading
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© 2001 Humana Press Inc., Totowa, NJ
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Manthorpe, M. (2001). Neural Cell Counting. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:277
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DOI: https://doi.org/10.1385/1-59259-207-4:277
Publisher Name: Humana Press
Print ISBN: 978-0-89603-902-5
Online ISBN: 978-1-59259-207-4
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