Abstract
Although the methods given in Chapter 109 can give an approximate idea of oligosaccharide amount or composition, they would not be able to distinguish the multiple monosaccharides and substituents present in nature. For this, the high resolution of gas chromatography (GC) is required (1,2). The most unambiguous results are provided by analysis of trimethylsilyl ethers (TMS) of methyl glycosides with on line mass selective detection (MS).
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References
Hounsell, E. F. (1993) A general strategy for glycoprotein oligosaccharide analysis, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 1–15.
Hounsell, E. F. (1994) Physicochemical analyses of oligosaccharide determinants of glycoproteins. Adv. Carbohyd. Chem. Biochem. 50, 311–350.
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© 2002 Humana Press Inc., Totowa, NJ
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Hounsell, E.F., Davies, M.J., Smith, K.D. (2002). Monosaccharide Analysis by Gas Chromatography (GC). In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:809
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DOI: https://doi.org/10.1385/1-59259-169-8:809
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
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