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Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting

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The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Glycoproteins result from the covalent association of carbohydrate moieties (gly-cans) with proteins. The enzymatic glycosylation of proteins is a common and complex form of posttranslational modification. The precise roles played by the carbohydrate moieties of glycoproteins are beginning to be understood (1-3). It has been established that glycans perform important biological roles including: stabilization of the protein structure; protection from degradation; and control of protein solubility, protein transport in cells, and protein half-life in blood. They also mediate the interactions with other macromolecules and the recognition and association with viruses, enzymes, and lectins (4-6).

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Authors and Affiliations

  1. Triskel Integrated Services, Geneva, Switzerland

    Patricia Gravel

Authors
  1. Patricia Gravel
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 2002 Humana Press Inc., Totowa, NJ

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Gravel, P. (2002). Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:779

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  • DOI: https://doi.org/10.1385/1-59259-169-8:779

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-940-7

  • Online ISBN: 978-1-59259-169-5

  • eBook Packages: Springer Book Archive

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