Abstract
Since the initial publication by Towbin and coworkers (1) on the preparation of replicas of sodium dodecyl sulfate (SDS)-polyacrylamide gel patterns, commonly called protein blots, the technique of transferring proteins from inaccessible gel matrices to accessible solid supports, such as nitrocellulose or nylon membranes, has become widely utilized (2,3).
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References
Towbin, H. E., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354.
Gershoni, J. M. and Palade, G. E. (1983) Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Analyt. Biochem. 124, 396–405.
Bers, G. and Garfin, D. (1985) Protein and nucleic acid blotting and immunobiochemical detection. Biotechniques 3, 276–288.
Moremans, M., Daneels, G., Van Dijck, A., Langanger, G., and De Mey, J. (1984) Sensi-tive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J. Immunol. Meth. 74, 353–360.
Hsu, Y.-H. (1984) Immunogold for detection of antigen on nitrocellulose paper. Analyt. Biochem. 142, 221–225.
Burnette, W. N. (1981) Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detec-tion with antibody and radioiodinated protein A. Analyt. Biochem. 112, 195–203.
Harper, D. R., Liu, K.-M., and Kangro, H. O. (1986) The effect of staining on the immuno-reactivity of nitrocellulose bound proteins. Analyt. Biochem. 157, 270–274.
Hancock, K. and Tsang, V. C. W. (1983) India ink staining of proteins on nitrocellulose paper. Analyt. Biochem. 133, 157–162.
Root, D. D. and Wang, K. (1993) Silver-enhanced copper staining of protein blots. Analyt. Biochem. 209, 15–19.
Wojtkowiak, Z., Briggs, R. C., and Hnilica, L. S. (1983) A sensitive method for staining proteins transferred to nitrocellulose paper. Analyt. Biochem. 129, 486–489.
Bayer, E. A., Wilchek, M., and Skutelsky, E. (1976) Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex. FEBS Lett. 68, 240–244.
LaRochelle, W. J. and Froehner S. C. (1986) Immunochemical detection of proteins bio-tiny lated on nitrocellulose replicas. J. Immunol. Meth. 92, 65–71.
LaRochelle, W. J. and Froehner, S. C. (1990) Staining of proteins on nitrocellulose repli-cas, in Methods in Enzymology, vol. 184 (Wilchek, M. and Bayer, E., eds.), Academic, San Diego, CA, pp. 433–436.
Bio-Rad Biotin-Blot Protein Detection Kit Instruction Manual (1989) Bio-Rad Laborato-ries Lit. No. 171., Richmond, CA, pp. 1–11.
Didenko, V. V. (1993) Biotinylation of DNA on membrane supports: a procedure for prepa-ration and easy control of labeling of nonradioactive single-stranded nucleic acid probes. Analyt. Biochem. 213, 75–78.
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© 2002 Humana Press Inc., Totowa, NJ
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Santora, K.E., Nelson, S.A., Lewis, K.A., LaRochelle, W.J. (2002). Utilization of Avidin or Streptavidin-Biotin as a Highly Sensitive Method to Stain Total Protein on Membranes. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:415
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DOI: https://doi.org/10.1385/1-59259-169-8:415
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