Abstract
Nonradioactively labeled probes offer several advantages compared to radioactive ones, as they show long stability, high morphological resolution, and rapid developing time. There are different types of nonradioactive labeling methods available, although digoxigenin-labeled probes (1) have become the most widely used for investigation on animal tissue, as they offer the advantage of low background noise and increased sensitivity (2,3). Also, digoxigenin can be used to label either RNA, DNA, or oligonucleotide probes. There have been different opinions on the sensitivity of detection of digoxigenin probes, but it has been shown that the sensitivity of radiolabeled and non-radioactive probes is comparable (3).
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References
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© 2000 Humana Press Inc., Totowa, NJ
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Terenghi, G. (2000). Use of Digoxigenin-Labeled Probes on Tissue Sections. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:985
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DOI: https://doi.org/10.1385/1-59259-038-1:985
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-459-4
Online ISBN: 978-1-59259-038-4
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