Abstract
The time and labor required for generating nucleotide sequence information has been significantly reduced since the development of the polymerase chain reaction (PCR). PCR allows the production of sufficient amounts of DNA template in vitro, and consequently the time-consuming cloning procedure to obtain large enough quantities of DNA for sequencing is avoided. The PCR has transformed nucleotide sequencing into a method that can be considered even in routine diagnostics for sequencing large numbers of individual samples.
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References
Casanova, J.-L., Pannetier, C., Jaulin, C, and Kourilsky, P. (1990) Optimal conditions for directly sequencing double-stranded PCR products with Sequenase. Nucleic Acids Res. 18, 4028.
Gyllensten, U. B. and Erlich, H. (1988) Generation of single stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQ alpha-locus. Proc. Natl. Acad. Sci. USA 85, 7652–7656.
Stoflet, E. S., Koeberl, D. D., Sarkar, G., and Sommer, S. S. (1988) Genomic amplification with transcript sequencing. Science 239, 491–494.
Syvänen, A.-C., Bengtström, M., Tenhunen, J., and Söderlund, H. (1988) Quantification of polymerase chain reaction products by affinity-based hybrid collection. Nucleic Acids Res. 16,11,327-11,338.
Syvänen, A.-C., Aalto-Setälä, K., Kontula, K., and Söderlund, H. (1989) Direct sequencing of affinity-captured amplified human DNA: application to the detection of apolipoprotein E polymorphism. FEBS Lett. 258, 71–74.
Hultman, T., Ståhl, S. Homes, E., and Uhlén, M. (1989) Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17, 4937–4946.
Hultman, T., Bergh, S., Moks, T., and Uhlén, M. (1991) Bidirectional solid-phase sequencing of in vitro amplified plasmid DNA. Bio Techniques 10, 84–93.
Lagerkvist, A., Stewart, J., Lagerström-Fermér, M., and Landegren, U. (1994). Manifold sequencing: efficient processing of large sets of sequencing reactions. Proc. Natl. Acad. Sci. USA 91, 2245–2249.
Syvänen, A.-C and Söderlund, H. (1993) Quantification of polymerase chain reaction products by affinity-based collection. Methods Enzymol. 218, 474–490.
Bengtström M, Jungell-Nortamo, A., and Syvänen, A.-C. (1990) Biotinylation of oligonucleotides using a water soluble biotin ester. Nucleosides Nucleotides 9, 123–127.
Wu, R., Wu, N.-H., Hanna, Z., Georges, F., and Narang, S. (1984) In Oligonucleotide Synthesis: A Practical Approach (Gait, M. J., ed.), IRL Press, Oxford, UK, p. 135.
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© 2000 Humana Press Inc., Totowa, NJ
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Suomalainen, A., Syvanen, AC. (2000). Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:547
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DOI: https://doi.org/10.1385/1-59259-038-1:547
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-459-4
Online ISBN: 978-1-59259-038-4
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