Abstract
The dideoxy chain-termination method (1) involves enzymatic elongation of a oligonucleotide primer that is annealed to a single-stranded DNA template. Single-stranded DNA of bacteriophage M13 (2) or phagemid vectors (3) give the most consistently satisfactory sequencing data. However, denatured double-stranded plasmid DNA can also serve as a suitable template and can also yield several hundred nucleotides of sequence information per reaction.
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References
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© 2000 Humana Press Inc., Totowa, NJ
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Brewis, N. (2000). Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:493
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DOI: https://doi.org/10.1385/1-59259-038-1:493
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-459-4
Online ISBN: 978-1-59259-038-4
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