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Preparation and Analysis of DNA Sequencing Gels

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The Nucleic Acid Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded products of sequencing reactions. A 4–20% polyacrylamide gel is used, normally 0.4 mm thick and at least 40 cm in length.

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References

  1. Biggin, M. D., Gibson, T. J., and Hong, G. F. (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA 80, 3963–3965.

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  3. Reed, A. P., Kost, T. A., and Miller, T. J. (1986) Simple improvements in 35S dideoxy sequencing. BioTechniques 4, 306–308.

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  4. Wahls, W. P. and Kingzette, M. (1988) No runs, no drips, no errors: a new technique for sealing polyacrylamide gel electrophoresis apparatus. BioTechniques 6, 308–309.

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© 2000 Humana Press Inc., Totowa, NJ

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Theophilus, B.D.M. (2000). Preparation and Analysis of DNA Sequencing Gels. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:481

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  • DOI: https://doi.org/10.1385/1-59259-038-1:481

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-459-4

  • Online ISBN: 978-1-59259-038-4

  • eBook Packages: Springer Book Archive

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