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Extraction and Purification of Plasmid DNA

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The Nucleic Acid Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Plasmids may be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. Many of these are rapid, small-scale “minipreps” that may be used effectively for plasmid analysis and further manipulation. The methods differ primarily in the means by which cells are lysed.

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References

  1. Birnboim, H. C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513–1523.

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  2. Holmes, D. S. and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114, 193–197.

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  3. Maniatis, T., Fritsch, E. F., and Sambrook, J. (eds.) (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 368,369.

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© 2000 Humana Press Inc., Totowa, NJ

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Winstanley, C., Rapley, R. (2000). Extraction and Purification of Plasmid DNA. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:327

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  • DOI: https://doi.org/10.1385/1-59259-038-1:327

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-459-4

  • Online ISBN: 978-1-59259-038-4

  • eBook Packages: Springer Book Archive

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