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NMR Structural Biology Using Paramagnetic Lanthanide Probe

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Advanced Methods in Structural Biology

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Abstract

We describe the recent development in nuclear magnetic resonance (NMR) equipped with paramagnetic lanthanide probe. Paramagnetic lanthanide probe provides long-range (~40 Å) distance and angular information that can be exploited in structure determination of large proteins and their complexes, dynamics, ligand screening, and structure-based resonance assignment. Application of the paramagnetic lanthanide probe is not limited to metal-binding proteins but becoming general by the use of lanthanide-binding tags. We here illustrate the practical aspects of the experiments and analyses for the use of paramagnetic lanthanide probe. Applications to protein-protein and protein-ligand structure determination and ligand screening are also shown.

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Acknowledgments

We would like to thank Dr. Yoshihiro Kobashigawa for providing the data for Fig. 5. A part of this work was supported by the Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program, Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan

    Tomohide Saio

  2. Department of Structural Biology, Faculty of Advanced Life Science, Hokkaido University, N-21, W-11, Kita-ku, Sapporo, 001-0021, Japan

    Tomohide Saio & Fuyuhiko Inagaki

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  1. Tomohide Saio
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  2. Fuyuhiko Inagaki
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Correspondence to Fuyuhiko Inagaki .

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Editors and Affiliations

  1. Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) , Tsukuba, Ibaraki, Japan

    Toshiya Senda

  2. Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan

    Katsumi Maenaka

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Saio, T., Inagaki, F. (2016). NMR Structural Biology Using Paramagnetic Lanthanide Probe. In: Senda, T., Maenaka, K. (eds) Advanced Methods in Structural Biology. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56030-2_17

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  • DOI: https://doi.org/10.1007/978-4-431-56030-2_17

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