Abstract
Almost two decades had passed since the first biosensor based on surface plasmon resonance (SPR) had become commercially available. Among them, the Biacore is the most widely used SPR-based system. More than 10,000 papers, which reported the results obtained using the Biacore (GE Healthcare), had been published until 2015. The most notable progress in the Biacore in this decade is marked reduction of the noise level, which enabled acquisition of the thermodynamic parameters, application to the low molecular weight analytes, observation of the thermodynamic parameters for the activated state, and analysis using further complicate binding model. This chapter aims to provide guidance to users of SPR, with an emphasis on acquiring the thermodynamic parameters for the molecular interaction of two-state binding mechanism, the system exhibiting the interconversion between the transient and the stable complex. No attempt will be made to describe the routine operation and maintenance of the Biacore, as this is comprehensively described elsewhere (Nagata K, Handa H (eds), Real-time analysis of biomolecular interactions: application of BIACORE. Springer, Tokyo, 2000).
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Abbreviations
- AGE:
-
Advanced glycation end product
- CDR:
-
Complementarity-determining region
- GA:
-
Glycolaldehyde
- IgG:
-
Immunoglobulin G
- ITC:
-
Isothermal titration calorimeter
- RU:
-
Resonance unit
- scFv:
-
Single-chain variable fragment
- SPR:
-
Surface plasmon resonance
- VH :
-
Variable region of immunoglobulin heavy chain
- VL :
-
Variable region of immunoglobulin light chain
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Acknowledgments
This work was partly supported by the Grants-in-Aid for Scientific Research (YK and HM) and the Grant-in-Aid for Scientific Research on Innovative Areas (HM) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Kobashigawa, Y., Fukuda, N., Nakahara, Y., Morioka, H. (2016). Surface Plasmon Resonance. In: Senda, T., Maenaka, K. (eds) Advanced Methods in Structural Biology. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56030-2_13
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DOI: https://doi.org/10.1007/978-4-431-56030-2_13
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