Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms

  • Tomoyuki Takahashi
  • Tetsuya Hori
  • Yukihiro Nakamura
  • Takayuki Yamashita
Part of the Springer Protocols Handbooks book series (SPH)


The synapse plays pivotal roles in various neuronal functions, with its efficacy determining the opening and closing of neuronal circuits. Among the parameters that determine synaptic efficacy, presynaptic factors are of crucial importance. However, these factors are among those that are the least well studied. Patch-clamp recording from the calyx of Held presynaptic terminal, described in this chapter, has enabled us to address directly questions about the presynaptic regulatory mechanisms in synaptic transmission.


Presynaptic Terminal Membrane Capacitance Patch Pipette Access Resistance Personal Computer Monitor 



We thank Ervin Johnson for helpful comments and English-language editing.


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Copyright information

© Springer 2012

Authors and Affiliations

  • Tomoyuki Takahashi
    • 1
    • 2
  • Tetsuya Hori
    • 1
    • 2
  • Yukihiro Nakamura
    • 1
    • 2
  • Takayuki Yamashita
    • 1
    • 3
  1. 1.Okinawa Institute of Science and TechnologyKunigamiJapan
  2. 2.Department of Neurophysiology, Faculty of Life and Medical SciencesDoshisha UniversityKyotanabeJapan
  3. 3.Laboratory of Sensory Processing, Brain Mind Institute, Faculty of Life ScienceEcole Polytechnique Federale de Lausanne (EPFL)LausanneSwitzerland

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