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Whole-Cell Patch Method

  • Protocol
Patch Clamp Techniques

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

With the whole-cell mode of patch-clamp the membrane current is recorded from a cell under the voltage-clamp. The membrane potential response of a cell is also measured and manipulated under a current-clamp. The method enables one to correlate the macroscopic aspects of ion channels with their microscopic properties. It also demonstrates how these ion channels regulate the membrane potential. This chapter summarizes the basic concepts of whole-cell patch-clamp recording and the practical procedures and protocols used to examine currents through channels/transporters across entire cells.

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Authors and Affiliations

  1. Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Miyagi, Japan

    Hiromu Yawo

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  1. Hiromu Yawo
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Correspondence to Hiromu Yawo .

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Editors and Affiliations

  1. National Institute for Physiological Sciences National Institutes of Natural Sciences, Okazaki, Aichi, Japan

    Yasunobu Okada

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Yawo, H. (2012). Whole-Cell Patch Method. In: Okada, Y. (eds) Patch Clamp Techniques. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53993-3_3

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  • DOI: https://doi.org/10.1007/978-4-431-53993-3_3

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-53992-6

  • Online ISBN: 978-4-431-53993-3

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