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Whole-Cell Patch Method

  • Hiromu Yawo
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

With the whole-cell mode of patch-clamp the membrane current is recorded from a cell under the voltage-clamp. The membrane potential response of a cell is also measured and manipulated under a current-clamp. The method enables one to correlate the macroscopic aspects of ion channels with their microscopic properties. It also demonstrates how these ion channels regulate the membrane potential. This chapter summarizes the basic concepts of whole-cell patch-clamp recording and the practical procedures and protocols used to examine currents through channels/transporters across entire cells.

Keywords

Pipette Solution Tail Current Charge Movement Compensation Voltage Stray Capacitance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Department of Developmental Biology and NeuroscienceTohoku University Graduate School of Life SciencesSendaiJapan

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