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Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature

  • Makoto Tominaga
  • Kunitoshi Uchida
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Since the cloning of the first thermosensitive TRP channel, a capsaicin receptor TRPV1, in 1997, nine thermosensitive TRP channels have been identified. Thermosensitive TRP channels can be used to detect local temperatures by expression in HEK293 cells as a kind of biosensor, as described in  Chap. 21, with a patch-clamp method. Because analyses of the thermosensitive ion channels have not yet been widely performed, the thermosensitive ion channels and detailed methods for their analysis are introduced here.

Keywords

HEK293 Cell Temperature Threshold Heat Stimulus Cold Stimulus Flex Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (1997) The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 389:816–824PubMedCrossRefGoogle Scholar
  2. 2.
    Venkatachalam K, Montell C (2007) TRP channels. Annu Rev Biochem 76:387–417PubMedCrossRefGoogle Scholar
  3. 3.
    Tominaga M, Caterina MJ (2004) Thermo-sensation and pain. J Neurobiol 61:3–12PubMedCrossRefGoogle Scholar
  4. 4.
    Dhaka A, Viswanath V, Patapoutian A (2006) TRP ion channels and thermosensation. Annu Rev Neurosci 29:135–161PubMedCrossRefGoogle Scholar
  5. 5.
    Talavera K, Nilius B, Voets T (2008) Neuronal TRP channels: thermometers, pathfinders and life-savers. Trends Neurosci 31:287–295PubMedCrossRefGoogle Scholar
  6. 6.
    Hille B (2001) Ion channels of excitable membranes, 3rd edn. Sinauer, Sunderland, MAGoogle Scholar

Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Division of Cell SignalingOkazaki Institute for Integrative Bioscience (National Institute for Physiological Science), National Institutes of Natural SciencesOkazakiJapan
  2. 2.Department of Physiological SciencesThe Graduate University for Advanced StudiesOkazakiJapan

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