Temperature-Evoked Channel Activation: Simultaneous Detection of Ionic Currents and Temperature

  • Makoto Tominaga
  • Kunitoshi Uchida
Part of the Springer Protocols Handbooks book series (SPH)


Since the cloning of the first thermosensitive TRP channel, a capsaicin receptor TRPV1, in 1997, nine thermosensitive TRP channels have been identified. Thermosensitive TRP channels can be used to detect local temperatures by expression in HEK293 cells as a kind of biosensor, as described in  Chap. 21, with a patch-clamp method. Because analyses of the thermosensitive ion channels have not yet been widely performed, the thermosensitive ion channels and detailed methods for their analysis are introduced here.


HEK293 Cell Temperature Threshold Heat Stimulus Cold Stimulus Flex Point 
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Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Division of Cell SignalingOkazaki Institute for Integrative Bioscience (National Institute for Physiological Science), National Institutes of Natural SciencesOkazakiJapan
  2. 2.Department of Physiological SciencesThe Graduate University for Advanced StudiesOkazakiJapan

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