Patch-Clamp Techniques: General Remarks

Part of the Springer Protocols Handbooks book series (SPH)


The patch-clamp technique, invented by Erwin Neher and Bert Sakmann in 1976, was originally designed to detect the activity of single-ion channel proteins in the cell membrane. The technique is now widely used in the field of ion channel research for many purposes, such as to monitor changes in the total membrane current, intracellular voltage or cell membrane capacitance etc. in a living cell at high time resolution. We explain the basic principle of, and protocols for, the patch-clamp technique, including maneuvers for patch-clamp amplifier operation – basic knowledge required for applications explained in the following chapters.


Pipette Solution Patch Membrane Junction Potential Recording Mode Perforated Patch 
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© Springer 2012

Authors and Affiliations

  1. 1.Department of Cell PhysiologyNational Institute for Physiological Sciences, National Institutes of Natural SciencesOkazakiJapan
  2. 2.Section of Electron MicroscopyNational Institute for Physiological Sciences, National Institutes of Natural SciencesOkazakiJapan
  3. 3.National Institute for Physiological Sciences, National Institutes of Natural SciencesOkazakiJapan

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