Patch-Clamp Capacitance Measurements

  • Takeshi SakabaEmail author
  • Akaihiro Hazama
  • Yoshio Maruyama
Part of the Springer Protocols Handbooks book series (SPH)


Not only electrical conductance but also electrical capacitance of the cell membrane can be measured by patch-clamp techniques. Exocytotic events can be detected by recording changes in membrane capacitance. The membrane capacitance, which reflects the surface area of the plasma membrane, increases during an exocytotic process by fusion of secretory granules to the plasma membrane. In this chapter, we describe the patch-clamp method for measuring capacitance.


Direct Current Secretory Granule Series Resistance Capacitance Measurement Capacitance Change 
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We thank Andreas Neef and Raunak Sinha for their comments.


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Copyright information

© Springer 2012

Authors and Affiliations

  • Takeshi Sakaba
    • 1
    Email author
  • Akaihiro Hazama
    • 2
  • Yoshio Maruyama
    • 3
  1. 1.Graduate School of Brain ScienceDoshisha UniversityKizugawaJapan
  2. 2.Fukushima Medical UniversityFukushimaJapan
  3. 3.Department of PhysiologyTohoku University Graduate School of MedicineSendaiJapan

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