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Planar Lipid Bilayer Method for Studying Channel Molecules

  • Protocol
Patch Clamp Techniques

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The planar lipid bilayer method is another way to examine channel molecules functionally at the single-molecule level. In contrast to patch-clamping, channel molecules are isolated from various biological resources and are reconstituted into an artificial membrane that has a defined lipid composition. Various techniques have been developed, from the conventional painting method to liposome-patch clamping. In this chapter, underlying principles and technical details for forming the planar lipid bilayer and methods for incorporating channel molecules into the bilayer are reviewed.

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  1. Department of Molecular Physiology and Biophysics, School of Medicine, University of Fukui Faculty of Medical Sciences, Fukui, Japan

    Shigetoshi Oiki

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  1. Shigetoshi Oiki
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  1. National Institute for Physiological Sciences National Institutes of Natural Sciences, Okazaki, Aichi, Japan

    Yasunobu Okada

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Oiki, S. (2012). Planar Lipid Bilayer Method for Studying Channel Molecules. In: Okada, Y. (eds) Patch Clamp Techniques. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53993-3_16

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  • DOI: https://doi.org/10.1007/978-4-431-53993-3_16

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