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Pipette Perfusion Technique

  • Protocol
Book cover Patch Clamp Techniques

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The piette perfusion technique is a version of patch-clamp techniques and provides a greater intracellular access during electrophysiological recordings. This internal perfusion technique offers the intracellular change of not only ions but also substances with higher molecular weight such as enzymes, antibodies, and metabolites. As to the other versions of techniques, it requires dedicated time and personal training. This chapter describes the tips and tricks of the unique pipette perfusion technique.

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Authors and Affiliations

  1. Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Sciences, Otsu, Shiga, Japan

    Minoru Horie

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  1. Minoru Horie
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Correspondence to Minoru Horie .

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Editors and Affiliations

  1. National Institute for Physiological Sciences National Institutes of Natural Sciences, Okazaki, Aichi, Japan

    Yasunobu Okada

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© 2012 Springer

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Horie, M. (2012). Pipette Perfusion Technique. In: Okada, Y. (eds) Patch Clamp Techniques. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53993-3_15

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  • DOI: https://doi.org/10.1007/978-4-431-53993-3_15

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-53992-6

  • Online ISBN: 978-4-431-53993-3

  • eBook Packages: Springer Protocols

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