Giant Patch and Macro Patch

Part of the Springer Protocols Handbooks book series (SPH)


The giant patch method was first developed in 1989. The major characteristic is the use of a pipette with a large tip diameter, which dramatically broadened the applications of the excised patch. The giant patch method enables (1) the current recording of transporters/channels with slow turnover rates and low expression levels, which had been impossible with the conventional method; (2) the rapid voltage-clamp; (3) rapid exchange of the bath and pipette solutions. We describe the devices and limitations for the application of the giant patch method to cardiomyocytes, Xenopus oocytes, and cultured cells. In addition, we describe another method, the macro patch, which we developed to overcome one of the limitations of the cardiac giant patch.


Cystic Fibrosis Transmembrane Conductance Regulator Bath Solution Xenopus Oocyte Platinum Wire Ventricular Myocytes 
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  1. 1.
    Hilgemann DW (1989) Giant excised cardiac sarcolemmal membrane patches: sodium and sodium-calcium exchange currents. Pflugers Arch 415:247–249PubMedCrossRefGoogle Scholar
  2. 2.
    Hilgemann DW (1995) The giant membrane patch. In: Sakmann B, Neher E (eds) Single-channel recording, 2nd edn. Plenum, New YorkGoogle Scholar
  3. 3.
    Hilgemann DW, Lu CC (1998) Giant membrane patches: improvements and applications. Methods Enzymol 293:267–280PubMedCrossRefGoogle Scholar
  4. 4.
    Couey JJ, Ryan DP, Glover JT, Dreixler JC, Young JB, Houamed KM (2002) Giant excised patch recordings of recombinant ion channel currents expressed in mammalian cells. Neurosci Lett 329:17–20PubMedCrossRefGoogle Scholar
  5. 5.
    Fujioka Y, Komeda M, Matsuoka S (2000) Stoichiometry of Na+-Ca2+ exchange in inside-out patches excised from guinea-pig ventricular myocytes. J Physiol 523:339–351PubMedCrossRefGoogle Scholar
  6. 6.
    Fujioka Y, Hiroe K, Matsuoka S (2000) Regulation kinetics of Na+-Ca2+ exchange current in guinea-pig ventricular myocytes. J Physiol 529:611–623CrossRefGoogle Scholar
  7. 7.
    Collins A, Somlyo AV, Hilgemann DW (1992) The giant cardiac membrane patch method: stimulation of outward Na+-Ca2+ exchange current by MgATP. J Physiol 454:27–57PubMedGoogle Scholar
  8. 8.
    Hilgemann DW, Collins A (1992) Mechanism of cardiac Na+-Ca2+ exchange current stimulation by MgATP: possible involvement of aminophospholipid translocase. J Physiol 454:59–82PubMedGoogle Scholar
  9. 9.
    Doering AE, Lederer WJ (1994) The action of Na+ as a cofactor in the inhibition by cytoplasmic protons of the cardiac Na+-Ca2+ exchanger in the guinea-pig. J Physiol 480:9–20PubMedGoogle Scholar
  10. 10.
    Matsuoka S, Nicoll DA, Reilly RF, Hilgemann DW, Philipson KD (1993) Initial localization of regulatory regions of the cardiac sarcolemmal Na+-Ca2+ exchanger. Proc Natl Acad Sci USA 90:3870–3874PubMedCrossRefGoogle Scholar
  11. 11.
    Matsuoka S, Nicoll DA, Hryshko LV, Levitsky DO, Weiss JN, Philipson KD (1995) Regulation of the cardiac Na+-Ca2+ exchanger by Ca2+: mutational analysis of the Ca2+-binding domain. J Gen Physiol 105:403–420PubMedCrossRefGoogle Scholar
  12. 12.
    Soejima M, Noma A (1984) Mode of regulation of the ACh-sensitive K-channel by the muscarinic receptor in rabbit atrial cells. Pflugers Arch 400:424–431PubMedCrossRefGoogle Scholar
  13. 13.
    Hilgemann DW, Matsuoka S, Nagel GA, Collins A (1992) Steady-state and dynamic properties of cardiac sodium-calcium exchange: sodium-dependent inactivation. J Gen Physiol 100:905–932PubMedCrossRefGoogle Scholar
  14. 14.
    Qin DY, Noma A (1988) A new oil-gate concentration jump technique applied to inside-out patch-clamp recording. Am J Physiol 255:H980–H984PubMedGoogle Scholar
  15. 15.
    Friedrich T, Bamberg E, Nagel G (1996) Na+, K+-ATPase pump currents in giant excised patches activated by an ATP concentration jump. Biophys J 71:2486–2500PubMedCrossRefGoogle Scholar
  16. 16.
    Collins A, Hilgemann DW (1993) A novel method for direct application of phospholipids to giant excised membrane patches in the study of sodium-calcium exchange and sodium channel currents. Pflugers Arch 423:347–355PubMedCrossRefGoogle Scholar
  17. 17.
    Nagel G, Hwang TC, Nastiuk KL, Nairn AC, Gadsby DC (1992) The protein kinase A-regulated cardiac Cl channel resembles the cystic fibrosis transmembrane conductance regulator. Nature 360:81–84PubMedCrossRefGoogle Scholar
  18. 18.
    Hilgemann DW (1994) Channel-like function of the Na, K pump probed at microsecond resolution in giant membrane patches. Science 263:1429–1432PubMedCrossRefGoogle Scholar

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© Springer 2012

Authors and Affiliations

  1. 1.Department of Physiology and Biophysics, Graduate School of MedicineKyoto UniversityKyotoJapan
  2. 2.Center for Innovation in Immunoregulative Technology and Therapeutics, Graduate School of MedicineKyoto UniversityKyotoJapan

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