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Two-Photon Targeted Patch-Clamp Recordings In Vivo

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Patch Clamp Techniques

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Abstract

The advent of two-photon microscopy has enabled us to visualize individual neurons in the intact brain. This technique, used in combination with whole-cell patch-clamp recordings, has facilitated targeted intracellular recording from particular neurons of interest. This chapter provides a practical guide for implementing in vivo two-photon targeted patch-clamp recording and describes potential outcomes using the technique.

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Correspondence to Kazuo Kitamura .

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© 2012 Springer

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Kitamura, K. (2012). Two-Photon Targeted Patch-Clamp Recordings In Vivo. In: Okada, Y. (eds) Patch Clamp Techniques. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53993-3_12

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  • DOI: https://doi.org/10.1007/978-4-431-53993-3_12

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-53992-6

  • Online ISBN: 978-4-431-53993-3

  • eBook Packages: Springer Protocols

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