Abstract
Here an established method is described for performing the entire FISH procedure in suspension instead of on a slide, as usual. This so-called suspension FISH (S-FISH) opened new possibilities for the analysis of shape internal structure of the human interphase nucleus in different tissues starting. The procedure is described and some applications of this approach are presented.
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References
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Liehr, T., Kosyakova, N. (2017). Three-Dimensional Interphase Analysis Enabled by Suspension FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_41
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DOI: https://doi.org/10.1007/978-3-662-52959-1_41
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Online ISBN: 978-3-662-52959-1
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