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Interphase FISH for Detection of Chromosomal Mosaicism

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Fluorescence In Situ Hybridization (FISH)

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Abstract

Interphase fluorescence in situ hybridization (iFISH) allows qualitative and quantitative detection of chromosomal DNA at all stages of the cell cycle and at molecular resolutions. Accordingly, this methodology offers an opportunity to address chromosome numbers and structures in all the human tissues. In this light, a variety of iFISH techniques have been acknowledged as indispensable for studying intercellular genomic variation or somatic chromosomal mosaicism. Here, an interphase FISH protocol for the detection of intercellular genomic variations and low-level chromosomal mosaicism in somatic tissues is described. Additionally, technical issues influencing the results of iFISH are considered.

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Acknowledgments

The chapter is dedicated to Ilia V. Soloviev. The authors are supported by a grant from the Russian Science Foundation (project #14-35-00060).

Author information

Authors and Affiliations

  1. Mental Health Research Center, Moscow, Russia

    Ivan Y. Iourov, Svetlana G. Vorsanova & Yuri B. Yurov

  2. Russian National Research Medical University named after N. I. Pirogov, Separated Structural Unit “Clinical Research Institute of Pediatrics”, Ministry of Health of Russian Federation, Moscow, Russia

    Ivan Y. Iourov, Svetlana G. Vorsanova & Yuri B. Yurov

  3. Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Moscow, Russia

    Ivan Y. Iourov

  4. Moscow State University of Psychology and Education, Moscow, 127051, Russia

    Svetlana G. Vorsanova & Yuri B. Yurov

Authors
  1. Ivan Y. Iourov
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  2. Svetlana G. Vorsanova
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  3. Yuri B. Yurov
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Corresponding author

Correspondence to Ivan Y. Iourov .

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Editors and Affiliations

  1. Institut für Humangenetik, Jena, Germany

    Thomas Liehr

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Iourov, I.Y., Vorsanova, S.G., Yurov, Y.B. (2017). Interphase FISH for Detection of Chromosomal Mosaicism. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_38

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  • DOI: https://doi.org/10.1007/978-3-662-52959-1_38

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