Skip to main content

FISH Banding Techniques

  • Protocol
  • First Online:
Fluorescence In Situ Hybridization (FISH)

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 3172 Accesses

Abstract

During the last decade, numerous chromosome banding techniques based on FISH were developed for the human and for the murine genome. Here we review FISH banding techniques, which were recently defined as “any kind of FISH-technique, which provide the possibility to characterize simultaneously several chromosomal subregions smaller than a chromosome arm” and are DNA specific. FISH banding methods are successfully applied in research and diagnostics.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Claussen U, Michel S, Mühlig P et al (2002) Demystifying chromosome preparation and the implications for the concept of chromosome condensation during mitosis. Cytogenet Genome Res 98:136–146

    Article  CAS  PubMed  Google Scholar 

  2. Speicher MR, Gwyn Ballard S, Ward DC (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet 12:368–375

    Article  CAS  PubMed  Google Scholar 

  3. Schröck E, du Manoir S, Veldman T et al (1996) Multicolor spectral karyotyping of human chromosomes. Science 273:494–497

    Article  PubMed  Google Scholar 

  4. Liehr T, Heller A, Starke H et al (2002) FISH banding methods: applications in research and diagnostics. Expert Rev Mol Diagn 2:217–225

    Article  CAS  PubMed  Google Scholar 

  5. Liechty MC, Carpio CM, Aytay S et al (1999) Hybridization-based karyotyping of mouse chromosomes: hybridization-bands. Cytogenet Cell Genet 86:34–38

    Article  CAS  PubMed  Google Scholar 

  6. Liechty MC, Clase AC, Puschus KL et al (2000) Mouse linkage cytogenetics (L-C) probes. Cytogenet Cell Genet 88:163–167

    Article  CAS  PubMed  Google Scholar 

  7. Henegariu O, Dunai J, Chen XN et al (2001) A triple color FISH technique for mouse chromosome identification. Mamm Genome 12:462–465

    Article  CAS  PubMed  Google Scholar 

  8. Benedek K, Chudoba I, Klein G et al (2004) Rearrangements of the telomeric region of mouse chromosome 11 in Pre-B ABL/MYC cells revealed by mBANDing, spectral karyotyping, and fluorescence in-situ hybridization with a subtelomeric probe. Chromosome Res 12:777–785

    Article  CAS  PubMed  Google Scholar 

  9. Leibiger C, Kosyakova N, Mkrtchyan H et al (2013) First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding. J Histochem Cytochem 61:306–312

    Article  PubMed  PubMed Central  Google Scholar 

  10. Aurich-Costa J, Vannier A, Gregoire E et al (2001) IPM-FISH, a new M-FISH approach using IRS-PCR painting probes: application to the analysis of seven human prostate cell lines. Genes Chromosomes Cancer 30:143–160

    Article  CAS  PubMed  Google Scholar 

  11. Müller S, Rocchi M, Ferguson-Smith MA et al (1997) Toward a multicolor chromosome bar code for the entire human karyotype by fluorescence in situ hybridization. Hum Genet 100:271–278

    Article  PubMed  Google Scholar 

  12. Müller S, O’Brien PC, Ferguson-Smith MA et al (1998) Cross-species colour segmenting: a novel tool in human karyotype analysis. Cytometry 33:445–452

    Article  PubMed  Google Scholar 

  13. Weise A, Heller A, Starke H et al (2003) Multitude multicolor chromosome banding (mMCB)—a comprehensive one-step multicolor FISH banding method. Cytogenet Genome Res 103:34–39

    Article  CAS  PubMed  Google Scholar 

  14. Chudoba I, Plesch A, Lörch T et al (1999) High resolution multicolor banding: a new technique for refined FISH analysis of human chromosomes. Cytogenet Cell Genet 84:156–160

    Article  CAS  PubMed  Google Scholar 

  15. Liehr T, Heller A, Starke H et al (2002) Microdissection based high resolution multicolor banding for all 24 human chromosomes. Int J Mol Med 9:335–339

    CAS  PubMed  Google Scholar 

  16. Kakazu N, Ashihara E, Hada S et al (2001) Development of spectral colour banding in cytogenetic analysis. Lancet 357:529–530

    Article  CAS  PubMed  Google Scholar 

  17. Kakazu N, Bar-Am I, Hada S et al (2003) A new chromosome banding technique, spectral color banding (SCAN), for full characterization of chromosomal abnormalities. Genes Chromosomes Cancer 37:412–416

    Article  CAS  PubMed  Google Scholar 

  18. Tjia WM, Sham JS, Hu L et al (2005) Characterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes. Cancer Genet Cytogenet 158:61–66

    Article  CAS  PubMed  Google Scholar 

  19. Lichter P, Tang CJ, Call K et al (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69

    Article  CAS  PubMed  Google Scholar 

  20. Lengauer C, Green ED, Cremer T (1992) Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. Genomics 13:826–828

    Article  CAS  PubMed  Google Scholar 

  21. Liehr T, Weise A, Heller A et al (2002) Multicolor chromosome banding (MCB) with YAC/BAC-based probes and region-specific microdissection DNA libraries. Cytogenet Genome Res 97:43–50

    Article  CAS  PubMed  Google Scholar 

  22. Liehr T (2016) Multicolor FISH homepage. http://ssmc-tl.com/mfish.html

  23. Liehr T, Starke H, Weise A et al (2004) Multicolor FISH probe sets and their applications. Histol Histopathol 19:229–237

    CAS  PubMed  Google Scholar 

  24. Weise A, Mrasek K, Fickelscher I et al (2008) Molecular definition of high resolution multicolor banding (MCB) probes—first within the human DNA-sequence anchored FISH-banding probe set. J Histochem Cytochem 56:487–493

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Thomas Liehr .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer-Verlag Berlin Heidelberg

About this protocol

Cite this protocol

Liehr, T., Kosyakova, N., Weise, A. (2017). FISH Banding Techniques. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_26

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-52959-1_26

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-52957-7

  • Online ISBN: 978-3-662-52959-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics