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Transgenic Production Benchmarks

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Advanced Protocols for Animal Transgenesis

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The efficiency with which transgenic mice can be produced via pronuclear injection of DNA constructs is subject to a large number of variables ranging from human to mechanical to biological. Transgenic core facilities, which are often run like small businesses that must attract and satisfy clients, would benefit from knowing how their efficiency compares to that of other facilities, and whether significant improvements in any phase of the process can be realistically achieved. Communicating knowledgeably with clients about the amount of variation to expect at each step is also important in maintaining good working relationships. We describe a world-wide survey of transgenic core facilities designed to estimate the average yields that can be expected at each step in the process, as well as the inherent variability at each step. The survey has been conducted with the support of the International Society for Transgenic Technologies (ISTT). Some descriptive statistics, calculated from a subset of the data collected so far, are presented for different strains of mice and types of DNA constructs.

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Author information

Authors and Affiliations

  1. University of California, Irvine, CA, 92697–1310, USA

    Thomas J. Fielder

  2. University Laboratory Animal Resources, Irvine, CA, 92697–1310, USA

    Thomas J. Fielder

  3. Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, C/Darwin 3, 28049, Madrid, Spain

    Lluis Montoliu

  4. Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), ISCIII, Madrid, Spain

    Lluis Montoliu

Authors
  1. Thomas J. Fielder
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  2. Lluis Montoliu
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Corresponding author

Correspondence to Thomas J. Fielder .

Editor information

Editors and Affiliations

  1. Div. Biology, California Institute of Technology, Pasadena, 91125, California, USA

    Shirley Pease

  2. Transgenic Animal Model Core, University of Michigan, Ann Arbor, 48109-5674, Michigan, USA

    Thomas L. Saunders

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© 2011 Springer-Verlag Berlin Heidelberg

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Fielder, T.J., Montoliu, L. (2011). Transgenic Production Benchmarks. In: Pease, S., Saunders, T. (eds) Advanced Protocols for Animal Transgenesis. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20792-1_5

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  • DOI: https://doi.org/10.1007/978-3-642-20792-1_5

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-20791-4

  • Online ISBN: 978-3-642-20792-1

  • eBook Packages: Springer Protocols

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