Abstract
The scFv-chain Fv (scFv) corresponds to the smallest functional binding unit of a classical antibody and constitutes a favourite format for the comparative first evaluation of the recombinant potential of an antibody. In this protocol, a two-step cloning strategy is described following the modular exchange principle as a general guideline for converting antibodies into an scFv format suitable for periplasmatic expression in E. coli.
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© 2010 Springer-Verlag Berlin Heidelberg
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Müller, D. (2010). scFv by Two-Step Cloning. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_4
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DOI: https://doi.org/10.1007/978-3-642-01147-4_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-01146-7
Online ISBN: 978-3-642-01147-4
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