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Imaging Tumor Xenografts Using Radiolabeled Antibodies

  • Protocol
Antibody Engineering

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Non-invasive, molecular imaging of malignancies using radiolabeled antibodies as tracers is an emerging field that can offer information on staging and response to therapy. Protein engineering approaches enable fully human antibodies to be generated against any novel target and reformatted for optimal properties for positron emission tomography imaging (immunoPET). This chapter describes the steps required for imaging tumor xenografts in vivo in preclinical models with engineered antibody fragments. Two radiolabeling approaches, iodination and radiometal labeling, are described in detail including the conjugation of the radiometal chelator, DOTA, to the protein. Protocols for the assessment of the biological function of the protein after radiolabeling as well as establishment of tumor xenografts have also been included.

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Authors and Affiliations

  1. Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California Los Angeles, 570 Westwood Plaza, Los Angeles, CA, 90095, USA

    Tove Olafsen, Vania E. Kenanova & Anna M. Wu

Authors
  1. Tove Olafsen
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  2. Vania E. Kenanova
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  3. Anna M. Wu
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Corresponding author

Correspondence to Tove Olafsen .

Editor information

Editors and Affiliations

  1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Roland Kontermann

  2. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Spielmannstraße 7, 38106, Braunschweig, Germany

    Stefan Dübel

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© 2010 Springer-Verlag Berlin Heidelberg

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Olafsen, T., Kenanova, V.E., Wu, A.M. (2010). Imaging Tumor Xenografts Using Radiolabeled Antibodies. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_37

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  • DOI: https://doi.org/10.1007/978-3-642-01147-4_37

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01146-7

  • Online ISBN: 978-3-642-01147-4

  • eBook Packages: Springer Protocols

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