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Antibody Engineering pp 301–315Cite as

Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L

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Abstract

Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceuticals in clinical trials and their number is expanding rapidly (DataMonitor 2007). The antibodies or functional antibody fragments are being produced in artificial production systems like mammalian cells, yeast, bacteria and plant cells but also in transgenic animals like goats, sheep and cows. Regardless of the production method, the quality control demand is the same for all of them. Host cell proteins, cell culture media additives, DNA and endotoxins have to be removed from the mAb preparation to allow the proteins to be safely applied for human therapy. Moreover, antibody aggregates, clipped and low molecular weight species should also be removed.

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Authors and Affiliations

  1. Affitech AS, Gaustadalléen21, Oslo, 3490, Norway

    Remko Griep & John McDougall

Authors
  1. Remko Griep
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  2. John McDougall
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Correspondence to Remko Griep .

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Editors and Affiliations

  1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Roland Kontermann

  2. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Spielmannstraße 7, 38106, Braunschweig, Germany

    Stefan Dübel

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Griep, R., McDougall, J. (2010). Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_24

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  • DOI: https://doi.org/10.1007/978-3-642-01147-4_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01146-7

  • Online ISBN: 978-3-642-01147-4

  • eBook Packages: Springer Protocols

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