Except for when individuals have variations in the pericentric heterochromatic chromosomal regions (including acrocentric short arms), it is not possible to distinguish between homologous chromosomes at a single-cell level. Due to this limitation, various questions of scientific and diagnostic relevance have been off-limits. Based on copy number variations (CNV) spanning up to several megabasepairs of DNA, we developed a molecular cytogenetic approach for the interindividual differentiation of homologous chromosomes, the so-called parental origin determination fluorescence in situ hybridization (pod-FISH) technique. To perform this technique, all human chromosomes are covered with CNV-spanning BAC probes in one- to five-color chromosome-specific pod-FISH sets. This new approach to studying the parental origin of individual human chromosomes at a single-cell level has opened new horizons for diagnostics and basic research.
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Acknowledgments
Supported in part by a grant from the university of Jena, Deutsche Krebshilfe (70-3125-Li1), INTAS (AISbl 03-51-4060), IZKF Jena (Start-up S16), DFG (436 ARM 17/5/06), IZKF together with the TMWFK (TP 3.7 and B307-04004), Ernst-Abbe-Stiftung, Stiftung Leukämie, Stefan-Morsch-Stiftung, and Evangelische Studienwerk e.V. Villigst.
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Weise, A. et al. (2009). Parental Origin Determination FISH: Pod-FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_26
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DOI: https://doi.org/10.1007/978-3-540-70581-9_26
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